Interactions Tab, Feature-Level¶
The Interactions Tab provides experimentally and computationally derived host-pathogen protein-protein interaction data and visualizations from more than 15 public repositories, including STRINGDB, with a total of 55 million protein-protein interactions. Interactions are available at both the genome and feature levels, and are presented in tabular and graph format.
Accessing the Feature-Level Interactions Table on the PATRIC Website¶
Clicking the Interactions Tab in a Feature View displays the Interactions Table, listing of all of the interactions in PATRIC associated with that feature.
The tables include the following columns (fields) for each interaction, and are based on PSI-MITAB format:
- Interactor A: PATRIC ID (unique primary identifier) for one of the two interacting features (protein-coding genes).
- Gene A: Gene name for the feature, if available.
- Desc A: Protein product (functional annotation) associated with the feature.
- Interactor B: PATRIC ID for the other of the two interacting features.
- Gene B: Gene name for the feature, if available.
- Desc B: Protein product associated with the feature.
- Category: Category of interaction:
- PPI = protein-protein interaction
- HPI = host-pathogen interaction
- Interaction Type: Interaction types, taken from the corresponding PSI-MI controlled vocabulary.
- Detection Method: Interaction detection method, taken from the corresponding PSI-MI controlled vocabulary.
- Evidence: Evidence of the interaction:
- Experimental = identified by laboratory method
- Computational = predicted by computational method
Clicking the Graph option at the top left of the table displays the Cytoscape-based Interactions Viewer.
In the graph, proteins (interactors) are displayed as nodes and the interactions between the nodes are displayed as lines. Scrolling with the mouse zooms in/out in the graph. Clicking and dragging with the mouse moves the graph in the display. Gene names, where available, are displayed on the nodes. Mousing over a node or line displays detailed information about that interactor/interaction. Clicking and dragging on a node moves it in the graph, allowing rearrangement for better visualization.
Additional options are available from the upper right area of the graph. These include the following:
- Export: Creates a png image file of the graph and makes it available for saving to disk.
- Subgraph: Auto-selects subgraphs with more than 5, 10, or 20 nodes, or auto-selection of the largest subgraph.
- Hub Protein: Auto-selects hub proteins with more than 5, 10, or 20 nodes, or auto-selection of the most connected hub
- Layout: Changes the layout of the display, with several options available